---
title: "81 Pre-seed Biotech Investors in the US (Nov 2026)"
last_updated: "2026-11-06"
rows: "81"
free_rows: "25"
source: "SEC filings"
canonical: "https://theinvestordatabase.com/lists/pre-seed-biotech-investors-us/"
---

# 81 pre-seed biotech investors in the US

We list 81 pre-seed biotech investors in the US as of November 2026: each has an SEC filing on file, and the list was last checked on 6 Nov 2026. The cities with the most head offices on this list are Boston (9), San Francisco (7) and Palo Alto (6).

- 81 investors
- 50 with a fund filing or dated deal in the last 12 months
- $73M median fund size on file (SEC Form D, 58 firms)
- 438 named decision-makers on file

## First 25 of 81 pre-seed biotech investors in the US

| Investor | Type | HQ | Stages (our research) | Focus (our research) | Latest fund or deal | Fund size | Checked | Profile |
|---|---|---|---|---|---|---|---|---|
| SOSV | VC firm | United States | Pre-seed | Biotech, Deep Tech, Climate Tech, Healthtech | Portfolio news, Nov 2026 | $131M (SEC Form D, 2022) | 2026-11-04 | https://theinvestordatabase.com/investors/sosv/ |
| First In Ventures | VC firm | Hobe Sound, FL | Pre-seed, Seed | Biotech, Healthtech, AI, Climate Tech | New fund, Sep 2026 | $22M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/first-in-ventures/ |
| Transpose Platform | VC firm | Houston, TX | Pre-seed | Biotech, AI, Generalist | New fund, Sep 2026 | $500M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/transpose-platform/ |
| Madrona | VC firm | Seattle, WA | Pre-seed, Seed, Series A, Series B, Growth | Biotech, AI, Consumer, Deep Tech | New deal, Oct 2026 | $490M (SEC Form D, 2025) | 2026-11-05 | https://theinvestordatabase.com/investors/madrona/ |
| ARCH Venture Partners | VC firm | Chicago, IL | Pre-seed, Seed | Biotech, Healthtech, Deep Tech | New fund, Aug 2026 | $3.0B (SEC Form D, 2024) | 2026-11-04 | https://theinvestordatabase.com/investors/arch-venture-partners/ |
| Bison Ventures | VC firm | San Francisco, CA | Pre-seed, Seed, Series A, Series B | Biotech, AI, Healthtech, Energy | New fund, Oct 2026 | $160M (SEC Form D, 2025) | 2026-11-04 | https://theinvestordatabase.com/investors/bison-ventures/ |
| Avalon BioVentures | VC firm | La Jolla, CA | Pre-seed | Biotech, Healthtech, Space | Portfolio news, Nov 2024 | $200M (SEC Form D, 2021) | 2026-11-05 | https://theinvestordatabase.com/investors/avalon-bioventures/ |
| True Ventures | VC firm | Palo Alto, CA | Pre-seed, Seed | Biotech, AI, Fintech, Deep Tech | New fund, Sep 2026 | $800M (SEC Form D, 2022) | 2026-11-04 | https://theinvestordatabase.com/investors/true-ventures/ |
| 2048 Ventures | VC firm | Princeton, NJ | Pre-seed, Seed | Biotech, AI, Deep Tech, Healthtech | New deal, Jun 2026 | $82M (SEC Form D, 2025) | 2026-11-04 | https://theinvestordatabase.com/investors/2048-ventures/ |
| Cybernetix Ventures | VC firm | Boston, MA | Pre-seed, Seed, Series A | Biotech, Robotics, Industrial Tech, Logistics | New fund, Aug 2026 | $100M (SEC Form D, 2025) | 2026-11-04 | https://theinvestordatabase.com/investors/cybernetix-ventures/ |
| Thirdrockventures | VC firm | Boston, MA | Pre-seed, Seed | Biotech, Healthtech, Medtech | New fund, Jun 2026 | $1.1B (SEC Form D, 2022) | 2026-11-04 | https://theinvestordatabase.com/investors/thirdrockventures/ |
| Column Group | VC firm | San Francisco, CA | Pre-seed | Biotech, Healthtech | New deal, Jan 2026 | $600M (SEC Form D, 2022) | 2026-11-04 | https://theinvestordatabase.com/investors/column-group/ |
| How Women Invest | VC firm | Oakland, CA | Pre-seed, Seed, Series A, Series B | Biotech, Healthtech, AI, B2B | New fund, Sep 2026 | $12M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/how-women-invest/ |
| Atlas Venture | VC firm | Cambridge, MA | Pre-seed | Biotech, Healthtech | New deal, Jan 2026 | $450M (SEC Form D, 2022) | 2026-11-04 | https://theinvestordatabase.com/investors/atlas-venture/ |
| Mission BioCapital | VC firm | Cambridge, MA | Pre-seed | Biotech, Healthtech, Medtech | New fund, Apr 2026 | $192M (SEC Form D, 2021) | 2026-11-04 | https://theinvestordatabase.com/investors/mission-biocapital/ |
| Voltage Ventures | VC firm | Portland, ME | Pre-seed | Biotech, Healthtech, Medtech | New fund, Jul 2026 | $18M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/voltage-ventures/ |
| Hyve Ventures | VC firm | Austin, TX | Pre-seed | Biotech, Consumer, Healthtech, Logistics | New fund, Nov 2026 | $6M (SEC Form D, 2025) | 2026-11-04 | https://theinvestordatabase.com/investors/hyve-ventures/ |
| Monograph Capital | VC firm | Fort Worth, TX | Pre-seed | Biotech, Healthtech | New deal, Jun 2026 | $326M (SEC Form ADV) | 2026-11-05 | https://theinvestordatabase.com/investors/monograph-capital/ |
| 3i Partners | VC firm | Los Altos, CA | Pre-seed, Seed | Biotech, Mobility, Healthtech, Deep Tech | New fund, May 2026 | $24M (SEC Form D, 2025) | 2026-11-05 | https://theinvestordatabase.com/investors/3i-partners-los-altos/ |
| Draper Associates | VC firm | San Mateo, CA | Pre-seed, Seed, Series A | Biotech, AI, Fintech, Healthtech | New fund, Oct 2026 | $301M (SEC Form D, 2025) | 2026-11-04 | https://theinvestordatabase.com/investors/draper-associates/ |
| Even One Ventures | VC firm | San Francisco, CA | Pre-seed | Biotech, Healthtech, Medtech | New fund, Feb 2026 | $20M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/even-one-ventures/ |
| Cure Ventures | VC firm | Boston, MA | Pre-seed, Seed | Biotech, Healthtech | New fund, Dec 2025 | $350M (SEC Form D, 2021) | 2026-11-04 | https://theinvestordatabase.com/investors/cure-ventures/ |
| K5 Global | VC firm | Miami Beach, FL | Pre-seed, Growth, Any stage | Biotech, AI, Fintech, Healthtech | New fund, Oct 2026 | $10M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/k5-global/ |
| Averin | VC firm | Andover, MA | Pre-seed | Biotech, Healthtech, AI, Robotics | New fund, Mar 2026 | $350M (SEC Form D, 2026) | 2026-11-04 | https://theinvestordatabase.com/investors/averin/ |
| Kapor Capital | VC firm | Oakland, CA | Pre-seed, Seed, Series A | Biotech, Edtech, Fintech, Healthtech | New deal, Jun 2026 | $125M (SEC Form D, 2021) | 2026-11-05 | https://theinvestordatabase.com/investors/kapor-capital/ |

You are seeing 25 of 81. The full list adds 56 more investors and 438 named decision-makers.

Get all 81 investors and 438 named contacts, $49, one-time: https://theinvestordatabase.com/buy/list/pre-seed-biotech-investors-us
This page: https://theinvestordatabase.com/lists/pre-seed-biotech-investors-us/

## Method and limits
- Source: All 81 rows carry an SEC filing (Form D or Form ADV). All 81 have a website we checked. Filing date and fund size are SEC data where a filing exists. Stage, sector and check size are our research.
- Inclusion rule: Organizations that back startups. Individuals, buyout funds and firms we found to be inactive are left out. Sector: the firm states biotech and life sciences as a focus on its own site, or we observed it in the firm's portfolio. Stage: the firm states that it invests at pre-seed.
- Order: Activity score out of 100: recency of the latest fund filing or dated deal (30: full marks within 3 months, then stepping down at 6, 12 and 24 months), share of the observed portfolio in this sector (20; half of that when the sector is stated only), stage focus (15: all of it when pre-seed is the only stage the firm states, split evenly when it states more), stated focus on the US (15: full marks when the firm names the US as where it invests, half when it says global), size of the fund on file on a log scale (12) and completeness of the public record (8). Stage and regional focus are our research, not an SEC figure. It is a measure of activity and fit, not of quality or returns.
- Fill rates: Of 81 rows: stage 81, sector 81, fund size 70, website 81. In the paid file: named decision-makers on 80 rows, firm email on 26, LinkedIn page on 45, check size on 7.

## Questions
### How many pre-seed biotech investors in the US are there?
We list 81 as of November 2026. 50 of the 81 have a fund filing or a dated deal in the last 12 months. The cities with the most are Boston (9), San Francisco (7) and Palo Alto (6).

### Which pre-seed biotech investors in the US raised a new fund in the last 12 months?
40 of the 81 have a fund filing dated in the last 12 months. The most recent on this list are Hyve Ventures (Nov 2026), Draper Associates (Oct 2026) and Pear Ventures (Oct 2026).

### What is the typical fund size of pre-seed biotech investors in the US?
The median fund size on file for this list is $73M, across the 58 firms with a size on an SEC Form D. The fund on file is each firm's main fund, which is not always its newest. The other 23 do not disclose a size there.

### How do I contact pre-seed biotech investors in the US?
The paid file holds 438 named decision-makers across 80 of these 81 and a firm email for 26. We publish the address a firm lists itself, never a personal email. Each free row links to a profile with the firm's website.

### Where does the data on pre-seed biotech investors in the US come from?
All 81 rows have an SEC filing on file (Form D or Form ADV). Filing date and fund size come from the filing. Stage, sector and check size are our research. Rows on this list were last checked on 6 Nov 2026 at the latest. The whole file is re-verified twice a year.